Detection of cytomegalovirus in urine of HIV-infected patients by DNA-DNA hybridization comparison with virus isolation, immunofluorescence and immunoperoxidase
Mem. Inst. Oswaldo Cruz
;
87(1): 99-102, jan.-mar. 1992. tab
Article
in English
| LILACS
| ID: lil-116289
RESUMO
Immunofluorescence and immunoperoxidase test directed against early viral antigens, and DNA-DNA hybridization were compared with viral isolation for their abilities to detect Cytomegalovirus (CVM) in the urine of 89 HIV infected patients. From the 100 urine samples collected, 70 were found positive by at least one method. Considering viral isolation as the "gold standard" technique, immunofluorescence and immunoperoxidase had a sensitivity of 92.3% and88% respectively, with a specificity in both cases of 95%. DNA-DNA hybridization showed a sensitivity of 90% but with lower (60%) specificity. All of the three assays were effective in detecting CVM from urine and the technical advantage of each is discussed.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Adjuvants, Immunologic
/
Fluorescent Antibody Technique
/
Cytomegalovirus
/
Hybridization, Genetic
/
Antibodies, Monoclonal
Type of study:
Diagnostic study
Limits:
Humans
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
1992
Type:
Article
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