Dot-dye-immunoassay for the diagnosis of schistosomiasis mansoni
Mem. Inst. Oswaldo Cruz
;
87(2): 187-90, abr.-jun. 1992. tab, ilus
Article
in English
| LILACS
| ID: lil-116303
ABSTRACT
A new serological assay dot-dye-immunoassay (dot-DIA) was evaluated for the diagnosis of schistosomiasis mansoni. This method consist of four steps:
(a) biding of antigens to a nitrocellulose membrane (NC); (b) blocking of free sites of the NC; (c) incubation in specific primary antibody; (d) detection of primary antibody reactivity by color development using second antibody coupled to textile dyes. Sera from 82 individuals, 61 with Schistosoma mansoni eggs in the stool and 21 stool negative were tested by ELISA, dot-ELISA, and dotDIA. A high level of agreement between the methods tested was observed for all sera tested ELISA x dot-ELISA 95.1%, ELISA x dot-DIA 92.7% and dot-ELISA x dot-DIA 97.6%. In this study, dot-DIA proved to be a feasible, sensitive, rapid and practical test for the diagnosis of shcistosomiasis
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Schistosomiasis mansoni
/
Enzyme-Linked Immunosorbent Assay
/
Immunoassay
/
Immunoenzyme Techniques
Type of study:
Diagnostic study
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
1992
Type:
Article
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