Molecular and biological diversity of HIV-1 in Brazil
Mem. Inst. Oswaldo Cruz
;
87(2): 249-55, abr.-jun. 1992. tab, ilus
Article
in English
| LILACS
| ID: lil-116313
RESUMO
To determine the genomic polymorphism and biological properties present in HIV-1 Brazilian isolates, were analyzed five viral isolates obtained from patients residing in Rio de Janeiro (P1 and P5), Säo Paulo (P3) and Bahia (P2 and P4) states. For each viral isolate in vitro characteristics such as replication rate, syncytium-inducing capacity and cell death were observed in lymphoblastoid (H9, CEM and peripheral blood mononuclear cells) as well as monocytoid (U937) cells. In addition, the evaluation of the restriction fragment lenght polymorphism of these isolates was also performed using a panel of endonucleases such as Hind III, Bgl II, Sac I, Pst I, Kpn I and Eco RI. One of the isolates (P1), showed the highest phenotypic and genotypic divergence, when compared to others. The results found suggest a HIV heterogeneity in Brazil similar to that already described in other regions of the world
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Polymorphism, Restriction Fragment Length
/
DNA Restriction Enzymes
/
Fluorescent Antibody Technique
/
Immunoenzyme Techniques
/
HIV-1
/
Antigens, Viral
Limits:
Humans
Country/Region as subject:
South America
/
Brazil
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
1992
Type:
Article
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