Replication of dengue viruses in mosquito cell cultures: a model from ultrastructural observations
Mem. Inst. Oswaldo Cruz
;
87(4): 565-74, out.-dez. 1992. ilus
Article
in English
| LILACS
| ID: lil-116372
RESUMO
Mosquito cell cultures infected with human sera from dengue-1 and dengue-2 outbreaks, started in Rio de Janeiro by 1986 and 1990 respectively, were examined by electron microscopy at different times post the infection of cell cultures. More information was obtained about cell penetration of virus particles in the presence or not of antibodies, their pathway inside the cells, replication mode and exit. Infectiveness of the virus at those different stages can only be attributed to the particles appearing inside the trans-Golgi vesicles; most of all newly formed virus particles remain inside the RER-derived cell vesicles or inside lysosomes, even during cell lysis. Groups of larges particles, 65-75 nm in diameter at dengue-2 infections, persist during cell passage. The large amounts of smooth membrane structures, as vesicles or tabules inside the RER are attributed to a cell response to viral infection
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Virus Replication
/
Dengue Virus
/
Disease Vectors
/
Flavivirus
/
Culicidae
Limits:
Animals
Country/Region as subject:
South America
/
Brazil
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
1992
Type:
Article
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