Toxoplasma gondii: a rapid method for the isolation of pure tachyzoites: preliminary characterization of its genome
Mem. Inst. Oswaldo Cruz
;
85(4): 429-34, Oct.-Dec. 1990. ilus
Article
in English
| LILACS
| ID: lil-127780
ABSTRACT
A rapid and simple technique for the purification of Toxoplasma gondii tachyzoites was developed. Highly purified parasites were obtained from the peritoneal exudates of infected mice by means of two consecutive discontinous sucrose gradients run at low speed (10,000xg, 30 min). Parasites obtained by this method conserved its biological activity. Hybridizations tudies with DNA from healthy mice and from purified tachyzoites preparations demonstrated that Toxoplasma gondii tachyzoites DNA could be obtained with better than 90 per cents purity. Preliminary studies with DNA endonucleases showed the presence in the tachyzoites genome of highly repetitives sequences
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Toxoplasma
/
DNA, Protozoan
/
Genome
Limits:
Animals
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
1990
Type:
Article
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