BHK cell growth on microcarriers in a bioreactor

RESUMO

BHK21C13 an VERO cells were cultivated on microcarriers in a 5 liters bioreactor. The BHK21C13 cell concentration increased gradually to a peak of 1.5 x 10(elevado à potência 6) cells/ml after 4 days of culture, and that of VERO cells reached a peak of 5 x 10(elevado à potência 5) cells/ml after 6 days of culture. The BHK21C13 cells were shown to grow faster than VERO cells, and after 4 days of culture, about 98//of microcarriers were totally covered of BHK21C13 cells, when only about 35//of them were totally covered of VERO cells. This procedure was found to be efficient to prepare these cell cultures in concentrations high enough to allow its utilization as a substract for virus growth and veterinarian or human vaccines production