Capillary zone electrophoresis separation of kinins using a novel laser fluorescence detector
Braz. j. med. biol. res
;
27(1): 11-9, jan. 1994. tab, ilus
Article
in English
| LILACS
| ID: lil-136487
ABSTRACT
1. Bradykinin, Lys-bradykinin, Met-Lys-bradykinin, des-Arg9- bradykinin and des-Arg1- bradykinin were separated by capillary zone electrophoresis in an apparatus constructed in our laboratory which utilizes a novel N2 pulsed laser-induced fluorescence detector. 2. Detection limits of 1.2 fmol for fluorescamine-derivatized bradykinin and 90 attomol for O-phthaldiadehyde-derivatized bradykinin were achieved. 3. This powerful analytical tool is described and its successful application to the measurements of bradykinin after enzymatic release from blood is documented
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Index:
LILACS (Americas)
Main subject:
Electrophoresis
/
Kinins
/
Lasers
Limits:
Animals
Language:
English
Journal:
Braz. j. med. biol. res
Journal subject:
Biology
/
Medicine
Year:
1994
Type:
Article
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