Properties of phosphorylated glial fibrillary acidic protein in brain slices of guinea pig, mouse and rat
Braz. j. med. biol. res
;
27(1): 81-9, jan. 1994. ilus
Article
in English
| LILACS
| ID: lil-136496
ABSTRACT
1. Brain micro-slices from guinea pig, mouse and rat were incubated in Krebs-Ringer Na- HEPES buffered medium containing [32P]-phosphate and characterized by immunoblotting with polyclonal antibody to glial fibrillary acidic protein (GFAP). 2. GFAP presented small differences in two-dimensional electrophoretic mobility. 3. The phosphorylation of GFAP was dependent on Ca2+ in the incubation medium in adult animals. 4. Both the immunocontent and level of phosphorylation of GFAP were higher in hippocampus than in cerebral cortex of all the three species
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Index:
LILACS (Americas)
Main subject:
Cerebral Cortex
/
Glial Fibrillary Acidic Protein
/
Hippocampus
Limits:
Animals
Language:
English
Journal:
Braz. j. med. biol. res
Journal subject:
Biology
/
Medicine
Year:
1994
Type:
Article
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