Un nuevo sustrato para la deteccón de anticuerpos antimicondriales en suero humano / A new substrate for detection of antimitochondrial antibodies in human serum
Rev. méd. Chile
;
122(8): 889-99, ago. 1994. tab, ilus
Article
in Spanish
| LILACS
| ID: lil-137952
RESUMO
This work describes a new model to detect antimitochondrial antibodies using indirect immunofluorescence on mouse sperm as substrate. As controls conventional substrates and mitochondrial protein immunoblots were esed. An intense fluorescent reaction was visualized in the mitochondrial sheet of mouse sperms allowing a straightforward diagnosis of positive sera. Sera coming from 10 patients with progressive systemic sclerosis, 12 patients with systemic lupus erythematosus and 17 patients with primary biliary cirrhosis were tested with this method, confirming results obtained with conventional tests that use indirect immunofluorescence and rat frozen kidney slices as substrate. The new method is simpler, more accurate and has a lower margin of error
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Index:
LILACS (Americas)
Main subject:
Scleroderma, Systemic
/
Liver Cirrhosis, Biliary
/
Lupus Erythematosus, Systemic
Type of study:
Diagnostic study
Language:
Spanish
Journal:
Rev. méd. Chile
Journal subject:
Medicine
Year:
1994
Type:
Article
/
Project document
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