The role of glycolipid C in the GPI biosynthetic pathway in Trypanosoma brucei bloodstream forms
Braz. j. med. biol. res
; 27(2): 121-6, Feb. 1994. ilus
Article
in En
| LILACS
| ID: lil-138274
Responsible library:
BR26.1
ABSTRACT
The glycosylphosphatidylinositol (GPI) biosynthetic pathway in Trypanosoma brucei bloodstream forms includes the formation of glycolipid C. This molecule is the inositol-acylated form of the GPI anchor precursor, glycolipid A. There is no evidence for the transfer of glycolipid C to protein in vivo and the role of glycolipid C is unclear. In this paper we show that glycolipid C is not synthesised in the presence of phenylmethylsulphonyl fluoride (PMSF) and that glycolipid C is not an obligatory intermediate on the pathway to the formation of glycolipid A. Using pulse-chase experiments we show that glycolipid A and glycolipid C are in a dynamic equilibrium and we suggest that only the forward reaction (glycolipid A conversion to glycolipid C) is inhibited by PMSF
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Index:
LILACS
Main subject:
Phosphatidylinositols
/
Trypanosoma brucei brucei
/
Glycolipids
Language:
En
Journal:
Braz. j. med. biol. res
Journal subject:
BIOLOGIA
/
MEDICINA
Year:
1994
Type:
Article
/
Congress and conference