Structures of four oligosaccharides derived from the glycoinositolphospholipid of Leishmania adleri

Routier, F; Mendonça-Previato, L; Previato, J. O; Jones, C; Wait, R

Routier, F; Mendonça-Previato, L; Previato, J. O; Jones, C; Wait, R.

Braz. j. med. biol. res ; 27(2): 211-7, Feb. 1994. ilus

Article in English | LILACS | ID: lil-138287

ABSTRACT

ABSTRACT

Glycoinositolphospholipids (GIPLs) were extracted from the trypanosomatid Leishmania adleri by hot phenol extraction and the carbohydrate moieties isolated after base cleavage. Purification of the crude oligosaccharides by high performance anion exchange (HPAE) chromatography yielded four fractions whose structures were determined by a combination of methylation analysis, fast atom bombardment (FAB) mass spectrometry and two-dimensional nuclear magnetic resonance (NMR) spectroscopy

Subject(s)

Animals; Glycosphingolipids/chemistry; Oligosaccharides/chemistry; Spectrometry, Mass, Fast Atom Bombardment; Trypanosomatina/chemistry; Carbohydrate Sequence; Glycosphingolipids/isolation & purification; Molecular Sequence Data; Oligosaccharides/isolation & purification; Magnetic Resonance Spectroscopy

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Index: LILACS (Americas) Main subject: Oligosaccharides / Glycosphingolipids / Spectrometry, Mass, Fast Atom Bombardment / Trypanosomatina Limits: Animals Language: English Journal: Braz. j. med. biol. res Journal subject: Biology / Medicine Year: 1994 Type: Article / Congress and conference

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