Initiation of primary cell cultures from embryos of the mosquitoes Anopheles albimanus and Aedes taeniorhynchus (Diptera: Culicidae)
Mem. Inst. Oswaldo Cruz
;
90(4): 547-51, jul.-ago. 1995. ilus, tab
Article
in English
| LILACS
| ID: lil-157312
ABSTRACT
Primary cell cultures were obtained from eggs of Anopheles albimanus and Aedes taeniorhynchus mosquitoes, vectors of human malaria and of Venezuelan equine encephalitis virus, respectively. The cellular growth of the An. albimanus cells began four weeks after explanting the embryonic tissues in MK/VP12 medium, supplemented with 15 por cento fetal bovine serum. The culture showed heterogeneous cellular morphology. With regard to the Ae. taeniorhynchus culture, growth occurred three weeks after initiating the culture in MM/VP12 medium. The majority of cells were small and round. Karyotypes were examined in the latter species.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Culture Media
/
Anopheles
Limits:
Animals
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
1995
Type:
Article
Similar
MEDLINE
...
LILACS
LIS