In vitro induced antibody production for the diagnosis of herpes simplex virus infection in immunosuppressed patients
Braz. j. med. biol. res
;
29(4): 485-7, Apr. 1996. tab
Article
in English
| LILACS
| ID: lil-163890
ABSTRACT
We developed and evaluated a specific test for herpes simplex virus (HSV) infection based on the secretion of HSV-specific antibodies by lymphocytes stimulated in vitro with HSV-1 antigens. The in vitro induced antibody production (IVIAP) test was used for the diagnosis of HSV infection in 43 seropositive selected subjects:
9 healthy subjects (controls), 30 symptomatic patients (26 of them immunocompromised and 4 immunocompetent) and 4 patients with varicella zoster infection. Anti-HSV antibodies were detected by an immune assay using an anti-human IgG peroxidase conjugate. The test showed a sensitivity of 93 per cent (15/16) and specificity of 92 per cent (1/13) which were confirmed by positive culture or clinical and laboratory follow-up. One AIDS patient had a false-negative result and one false-positive result (1/9) was obtained among the healthy subjects. All patients infected with varicella zoster virus were negative to the IVIAP test. The test is rapid, inexpensive, easy to interpret and can be used for the diagnosis of HSV infections, especially in immunocompromised patients.
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Index:
LILACS (Americas)
Main subject:
In Vitro Techniques
/
Herpes Simplex
Type of study:
Diagnostic study
Limits:
Humans
Language:
English
Journal:
Braz. j. med. biol. res
Journal subject:
Biology
/
Medicine
Year:
1996
Type:
Article
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