Non enzymatic liver cells isolation: citrate-perchlorate technique
Acta physiol. pharmacol. ther. latinoam
;
46(2): 144-8, 1996. ilus
Article
in English
| LILACS
| ID: lil-172320
ABSTRACT
In the present study we describe a non-enzymatic technique for the isolation of rat hepatocytes by perfusion of liver through portal vein. The perfusion media consist of 1 mM sodium percholorate. 5 mM sodium citrate, 10 mM glucose, 129 mM NaCl and 0.1 per cent bovine-serum albumin at pH 7.4. After purification through diatrizoate gradient, electron microscopical studies revealed that most of purified hepatocytes were well preserved and presented a normal ultrastructure, thus correlating with previous biochemical results. The present method enables the recovery of metabolically and morphologically normal hepatocytes.
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Index:
LILACS (Americas)
Main subject:
Cell Separation
/
Citrates
/
Sodium Compounds
/
Perchlorates
/
Liver
Limits:
Animals
Language:
English
Journal:
Acta physiol. pharmacol. ther. latinoam
Journal subject:
Pharmacology
/
Physiology
/
Therapeutics
Year:
1996
Type:
Article
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