Receptores estrogénicos: estandarización de un método para su demostración en muestras incluídas en parafina / Estrogen receptors: standardization of a method for it demonstration in paraffin included samples

ABSTRACT

Detecting estrogenic receptors on histological samples of breast tumors has a strong significance on the prognosis and treatment of this disease. The clasical detection method involves the incubation of the tissue with a monoclonal antibody from mouse directed against the estrogenic receptor (primary antibody) and a second incubation with another antibody that is directed against with an anti mouse I g G antibody. Afterwards this reaction is identified by an enzyme (generally horseradish peroxidase) that acquires a characteristic color that allows the identification of the cells that have the receptor. We demonstrate here that is possible to simplify the method modifyng qualitatively the treatment of the tissue samples before its exposure to the primary antibody and also shortening the incubation times with this antibody