Enzyme-linked thyroglobulin immunoassay

Matsumura, L. K; Kunii, I. S; Maciel, R. M. B; Endo, Y; Amino, N

Matsumura, L. K; Kunii, I. S; Maciel, R. M. B; Endo, Y; Amino, N.

Rev. Hosp. Säo Paulo Esc. Paul. Med ; 6(3/4): 87-90, July-Dec. 1995. ilus, graf

Article in English | LILACS | ID: lil-180111

ABSTRACT

ABSTRACT

A one-step enzyme linked sandwich immunoassay using Silicone rods coated with rabbit anti-human thyroglobulin anti-Tg) immunoglobulin G (Fab') conjugated with beta-D-galactosidase was established for the measurement of thyroglobulin in human sera. The volume of serum needed for the assay was 2 mul The sensitivity of the assay was 1.52 amol/tube, corresponding to O.5 ng/ml. The precision was proven by coefficients of variation: intra-assay, 7.0 to 9.1 per cent: inter-assay, 5.3 to 7.4 per cent. The correlation between this EIA and RIA was O.91, p < O.O1.

Subject(s)

Humans; Animals; Rabbits; Autoantibodies/blood; beta-Galactosidase/metabolism; Immunoglobulin G/analysis; Thyroglobulin/blood; Immunoenzyme Techniques; Immunoglobulins; Neoplasm Metastasis/diagnosis; Rabbits/immunology; Sensitivity and Specificity; Silicones

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Index: LILACS (Americas) Main subject: Autoantibodies / Thyroglobulin / Immunoglobulin G / Beta-Galactosidase Type of study: Diagnostic study Limits: Animals / Humans Language: English Journal: Rev. Hosp. Säo Paulo Esc. Paul. Med Journal subject: Medicine Year: 1995 Type: Article

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