Biology of amazonian anopheline: XX. Ontogeny of esterases, leucine aminopeptidase and alpha-glycerophosphate dehydrogenase in Anopheles (Nyssorhynchus) darlingi Root, 1926 (Diptera, Culicidae)
Rev. bras. biol
;
56(3): 591-8, ago. 1996. ilus
Article
in English
| LILACS
| ID: lil-182685
ABSTRACT
The esterases, leucine aminopeptidase and alpha-glycerophosphate dehydrogenase revealed modifications in gene expressions during the development of Anopheles darlingi. The esterases showed five activity bands, 1 and 2 being more deeply stained during the larval stages than in pupae or adults, esterases 3 and 4 more deeply stained in pupae and adults whereas esterase 5 was present throughout development. Leucine aminopeptidase showed five activity bands: LAP2 and LAP5 were characteristic of larvae, LAP3 was specific for pupae and adults, LAP4 was detected only in pupae, and LAP1 and LAP6 were detected in all stages. Alpha-Glycerophosphate dehydrogenase presented one activity band on starch gel whose intensity increased with development. Two activity bands were detected on polyacrylamide gel (alpha-GPDH1 and alpha-GPDH2) in 4th-instar larvae (old pigmented larvae) and this activity increased with development.
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Index:
LILACS (Americas)
Main subject:
Genetic Variation
/
Gene Expression
/
Esterases
/
Glycerolphosphate Dehydrogenase
/
Leucyl Aminopeptidase
/
Anopheles
Limits:
Animals
Language:
English
Journal:
Rev. bras. biol
Journal subject:
Biology
Year:
1996
Type:
Article
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