Specific identification of Biomphalaria tenagophila and Biomphalaria occidentalis populations by the low stringency polymerase chain reaction
Mem. Inst. Oswaldo Cruz
;
92(1): 101-6, Jan.-Feb. 1997. ilus, mapas, tab
Article
in English
| LILACS, SES-SP
| ID: lil-182865
RESUMO
Althrough Biomphalaria occidentalis and B. tenagophila are indisguishable on the basis of shell morphology and the majority of their genital organs, only the latter is susceptible to infection with Schistosoma mansoni. Thus, the identification of these species is fundamental to epidemiological studies of schistosomiasis. Here we describe a simple and rapid method for differentiating B. tenagophila from B. occidentalis based on low stringency polymere chain reaction and using a pair of primers specific for the amplification of the 18S rRNA gene. Analysis of the low stringency product profiles of populations of these snails from different geographical regions confirmed this approach as being applicable to the identification of B. tenagophila and B. occidentalis in cases where classical morphology is inconclusive.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Biomphalaria
Type of study:
Diagnostic study
Limits:
Animals
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Year:
1997
Type:
Article
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