Histochemical characterization of NADPH-diaphorase activity in area 17 of diurnal and nocturnal primates and rodents
Braz. j. med. biol. res
;
29(10): 1355-62, Oct. 1996. ilus, tab, graf
Article
in English
| LILACS
| ID: lil-186186
ABSTRACT
NADPH-diaphorase (NADPH-d) activity was studied comparatively in area 17 of four mammalian species, two primates and two rodents. Three brain hemispheres each from adult capuchin-monkeys, owl-monkeys, agoutis and guinea pigs were fixed with aldehyde fixatives by perfusion and 200 mum sections were submitted to NADPH-d histochemistry, using the indirect malic enzyme method. In all species studied the neuropil pattern of enzyme activity presented a clear layered appearance. In primates, histochemical staining was most intense in layer IVc, while in rodents the highest intensity of the neuropil reaction was in supragranular layers (II and III). Comparison of cell density in grey and white matter showed that the majority of NADPH-d-positive neurones were located in the white matter of primates but not of rodents. Since NADPH-d is a nitric oxide synthase the results are very important for comparative functional studies of neuromediators and their correlations with laminar and modular organization of area 17 of the mammalian brain.
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Index:
LILACS (Americas)
Main subject:
Vision, Ocular
/
Visual Cortex
/
NADPH Dehydrogenase
Limits:
Animals
Language:
English
Journal:
Braz. j. med. biol. res
Journal subject:
Biology
/
Medicine
Year:
1996
Type:
Article
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