Induction of mitogenesis by synthetic peptides related to the structure of human fibroblast growth factor-1
Ciênc. cult. (Säo Paulo)
; 48(5/6): 359-60, Sept.-Dec. 1996. tab
Article
in En
| LILACS
| ID: lil-186336
Responsible library:
BR1.1
RESUMO
The synthetic peptide Ac-WFVGLKKNGSSKRGPRT-NH2,related to the human FGF-1 sequence, was shown to be mitogenic upon Balb/c 3T3 fibroblasts in culture (ED50=10-20 muM) and to compete with the growth factor for cellular binding (ID50 = 30-50 muM). The results described suggest that the mitogenic activity of the peptide is dependent on the presence of the residues 122-127 (WFVGLK) in its structure. Also, its affinity for the cellular receptors seems to be dependent on the presence of residues that are important for FGF-heparin binding such as K127, K133 and R137.
Search on Google
Index:
LILACS
Main subject:
Peptides
/
Fibroblast Growth Factors
/
Mitosis
Limits:
Animals
Language:
En
Journal:
Ciênc. cult. (Säo Paulo)
Journal subject:
CIENCIA
Year:
1996
Type:
Article