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A simple PCR-based procedure for plague diagnosis
Rev. Inst. Med. Trop. Säo Paulo ; 38(5): 371-3, set.-out. 1996. ilus
Article in English | LILACS | ID: lil-186878
RESUMO
Triturados de bacos de animais infectados experimentalmente com Y. pestis, suspensos em salina foram fervidos e os sobrenadantes usados diretamente para amplificacao do PCR sem previa extracao do DNA. O limiar de deteccao pode ser aumentado por uma segunda etapa de amplificacao (Nested-PCR). Nao houve infectados usados como controle
Subject(s)
Full text: Available Index: LILACS (Americas) Main subject: Plague / Yersinia pestis / Polymerase Chain Reaction Type of study: Diagnostic study Limits: Animals Language: English Journal: Rev. Inst. Med. Trop. Säo Paulo Journal subject: Tropical Medicine Year: 1996 Type: Article

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Full text: Available Index: LILACS (Americas) Main subject: Plague / Yersinia pestis / Polymerase Chain Reaction Type of study: Diagnostic study Limits: Animals Language: English Journal: Rev. Inst. Med. Trop. Säo Paulo Journal subject: Tropical Medicine Year: 1996 Type: Article