Isolation and possible composition of glucosylceramides from Paracoccidioides brasiliensis
Braz. j. med. biol. res
;
29(11): 1441-4, Nov. 1996. ilus, tab
Article
in English
| LILACS
| ID: lil-187202
ABSTRACT
Twelve different species of neutral monohexosyl ceramides (CMHs) and two species of neutral monohexosyl ceramides were isolated form mycelium and yeast forms of Paracoccidioides brasiliensis, respectively, by a combination of ion-exchange chromatography, HPLC, and HPTLC. The glucosylceramides did not react with sera from patients with paracoccidioidomycosis (PCM). Carbohydrate analysis indicated that CMHs contain glucose. Analysis of (1)H-NMR and mass spectrometry data suggest that the structure of the CMHs is Glcpbeta1(Cer (mycelium forms present 12 different ceramides and yeast forms present 2 different ceramides). The composition of the lipid moieties was analyzed by negative fast atom bombardment mass spectrometry. No glycosphingolipid other than glucosylceramide was detected in P. brasiliensis.
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Index:
LILACS (Americas)
Main subject:
Paracoccidioides
/
Glucosylceramides
Limits:
Animals
Country/Region as subject:
South America
/
Brazil
Language:
English
Journal:
Braz. j. med. biol. res
Journal subject:
Biology
/
Medicine
Year:
1996
Type:
Article
/
Congress and conference
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