Diagnosis of dengue by using reverse transcriptase-polymerase chain reaction
Mem. Inst. Oswaldo Cruz
;
92(5): 595-600, Sept.-Oct. 1997. ilus, tab
Article
in English
| LILACS
| ID: lil-194199
ABSTRACT
A rapid identification of dengue viruses from clinical samples by using a nested reverse transcriptase-polymerase chain reaction (RT-PCR) procedure was carried out for diagnostic and epidemiological purposes. RT-PCR identified DEN-1 and DEN-2 viruses in 41 per cent (41/100) of previously confirmed cases and provided an accurate confirmation of DHF in four fatal cases. RT-PCR was also useful for detecting and typing dengue viruses in suspected cases, allowing a rapid identification of new serotypes in endemic areas.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Dengue
Type of study:
Diagnostic study
/
Prognostic study
Limits:
Humans
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
1997
Type:
Article
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