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Combined method for simultaneous morphology, immunophenotype and karyotype (MAC) in leukemias
São Paulo med. j ; 115(1): 1336-42, jan.-fev. 1997. ilus, tab
Article in English | LILACS | ID: lil-196638
RESUMO
In the present study, a combined method (CM) for attaining simultaneous identification of leukemic cell morphology, karyotype and immunophenotype has been evaluated in 21 patients with acute leukemia and 1 with CML in blast crisis were studied for morphology citochemistry, immunophenotype and karyotype. Karyotype was performed in a bone marrow sample by using conventional techniques. In each case, direct method (DM) and/or three cultures were tried. The CM consisted in separating a small part of the material resulting from any of the cultures or DM, preparing slides through cytospin and immunophenotyping through APAAP method using the same monoclonal antibodies (MoAb) as for diagnosis. In 14 cases, the metaphases proved positive to the MoAb in 4, the cells with abnormality had their origin defined; in other 4 the karyotype was normal preventing any identification; 6 cases had minimal abnormalities not visible through CM; and in two cases abnormal karyotypes were detected only in the cultures with GM-CSF. This study showed that CM is feasible in cases where evident numerical or structural chromosomal abnormalties are present.
Subject(s)
Full text: Available Index: LILACS (Americas) Main subject: Leukemia / Immunophenotyping / Karyotyping Limits: Adolescent / Adult / Child / Child, preschool / Female / Humans / Infant / Male Language: English Journal: São Paulo med. j Journal subject: Cirurgia Geral / Ciˆncia / Ginecologia / Medicine / Medicina Interna / Obstetr¡cia / Pediatria / Sa£de Mental / Sa£de P£blica Year: 1997 Type: Article

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Full text: Available Index: LILACS (Americas) Main subject: Leukemia / Immunophenotyping / Karyotyping Limits: Adolescent / Adult / Child / Child, preschool / Female / Humans / Infant / Male Language: English Journal: São Paulo med. j Journal subject: Cirurgia Geral / Ciˆncia / Ginecologia / Medicine / Medicina Interna / Obstetr¡cia / Pediatria / Sa£de Mental / Sa£de P£blica Year: 1997 Type: Article