Separation and mapping of chromosomes of parasitic protozoa
Mem. Inst. Oswaldo Cruz
;
92(6): 815-9, Nov.-Dec. 1997. ilus, tab
Article
in English
| LILACS
| ID: lil-197222
ABSTRACT
Many protozoan parasites represent an important group of human pathogens. Pulsed Field Gradient Gel Electrophoresis (PFGE) analysis has been an important tool fundamental genetic studies of parasites like Trypanosoma, Leishmania Giardia or the human malaria Plasmodium falciparum. We present PFGE conditions allowing a high resolution separation of chromosomes ranging from 500 to 4000 kb within a two day electrophoresis run. In addition, we present conditions for separating large chromosomes (2000-6000 kb) within 36 hr. We demontrate that the application of two dimentional PFGE (2D-PFGE) technique to parasite karyotypes is a very useful method for the analysis of dispersed gene families and comparative studies of the intrachomosomal genome organization.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Chromosome Mapping
/
Chromosomes
/
Eukaryota
Limits:
Animals
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
1997
Type:
Article
/
Congress and conference
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