The Trypanosoma cruzi genome project: nuclear karyotype and gene mapping of clone CL Brener
Mem. Inst. Oswaldo Cruz
;
92(6): 821-8, Nov.-Dec. 1997. ilus, tab
Article
in English
| LILACS
| ID: lil-197223
ABSTRACT
By using improved pulsed field gel electrophoresis conditions, the molecular karyotype of the reference chromosomal bands ranging in size from 0.45 to 3.5 Magabase pairs (Mbp) were resolved in a single run. The weighted sum of the chromosomal bands was approximately 87 Mbp. Chromoblots were hybridized with 39 different homologous probes, 13 of which identified single chromosomes. Several markers linkage and four different linkage groups were identified, each comprising two markers. Densitometric analysis suggests that most of the chromosomal bands contain two or more chromosomes representing either homologous chromosomes and/or heterologous chromosomes with similar sizes.
Full text:
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Index:
LILACS (Americas)
Main subject:
Trypanosoma cruzi
/
Chromosome Mapping
/
Clone Cells
/
Karyotyping
Limits:
Animals
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
1997
Type:
Article
/
Congress and conference
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