Large-scale production and purification of recombinant protein from an insect cell/baculovirus system in Erlenmeyer flasks: application to the chicken poly(ADP-ribose) polymerase catalytic domain
Braz. j. med. biol. res
;
30(8): 923-8, Aug. 1997. ilus
Article
in English
| LILACS
| ID: lil-197246
ABSTRACT
A simple and inexpensive shaker/Erlenmeyer flask system for largescale cultivation of insect cells is described and compared to a commercial spinner system. On the basis of maximum cell density, average population doubling time and overproduction of recombinant protein, a better result was obtained with a simpler and less expensive biorector consisting of Erlenmeyer flasks and an ordinary shaker waterbath. Routinely, about 90 mg of pure poly(ADP-ribose) polymerase catalytic domain was obtained for a total of 3 x 10(9) infected cells in three liters of culture.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Polynucleotide Adenylyltransferase
/
Poly Adenosine Diphosphate Ribose
/
In Vitro Techniques
/
Recombinant Proteins
/
Baculoviridae
/
ADP Ribose Transferases
/
Insecta
Limits:
Animals
Language:
English
Journal:
Braz. j. med. biol. res
Journal subject:
Biology
/
Medicine
Year:
1997
Type:
Article
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