Dengue virus: detection and pathogeny
Arch. med. res
;
25(2): 211-4, 1994. ilus
Article
in English
| LILACS
| ID: lil-198804
ABSTRACT
Sequences from a cDNA of dengue virus type 4 were cloned into transcription vectors. These sequences included the E, NS1, NS2A, NS2B, NS3 genes. RNA transcipts produced in vitro from these plasmids were used in hybridization assays to detect dengue viral sequences. With these RNA-probes we have been able to detect molecules of serotype-specific dengue 4 viral RNA. Moreover, the riboprobes detected viral sequences of other serotypes in the following order of sensitivity 4 > 2 > 3 > 1, and might be useful to differentiate serotypes
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Index:
LILACS (Americas)
Main subject:
Plasmids
/
Cells, Cultured
/
Dengue
/
Dengue Virus
Type of study:
Diagnostic study
Language:
English
Journal:
Arch. med. res
Journal subject:
Medicine
Year:
1994
Type:
Article
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