Some aspects of idiopatyc regulation in Chagas disease and human schistosomiasis
Ciênc. cult. (Säo Paulo)
;
46(5/6): 405-11, Sept.-Dec. 1994. tab, graf
Article
in English
| LILACS
| ID: lil-199871
RESUMO
We developed an approach for the examination of antiidiotypic cell-mediated reactivity during chronic human infections. Antibodies against Schistosoma mansoni egg antigens (anti-SEA) and against Trypanosoma cruzi epimastigotes (anti-EPI) prepared from pooled and individual sera from patients with either schistosomiasis mansoni or Chagas disease were immunoaffinity purified on appropriate columns and used to stimulate patients' T cells. These antibodies preparations and their F (ab)2 fragments stimulated in a dose-dependent assay the proliferations of peripheral blood mononuclear cells (PBMC) and T lymphocytes from some, but not all actively infected individuals. Anti-idiotypic T cells can recognize and respond to anti-SEA or anti-EPI idiotypes directly. We contend that the most likely explanation of this stimulations is that the idiotype expressed on these anti-SEA or anti-EPI antibodies are acting as immunogens to stimulate antiidiotypic T lymphocytes that develop during the course of the infection. Immunization of rabbits with these antibodies (anti-SEA or anti-EPI) preparations, followed by absorption of the rabbit antisera on absorbants of normal Ig, produced specific anti-id reagents. Use of these reagents in competitive ELISA distinguished the idiotypic expression on anti-SEA or anti-EPI preparations obtained from patients' sera with different clinical forms of schistosomiasis or Chagas disease, respectively. The anti-SEA Id-reactive T cells from S. mansoni infected patients were capable of regulating autologous in vitro granuloma formation.
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Index:
LILACS (Americas)
Main subject:
Schistosomiasis mansoni
/
Chagas Disease
/
Immunoglobulin Idiotypes
Limits:
Animals
/
Humans
Language:
English
Journal:
Ciênc. cult. (Säo Paulo)
Journal subject:
Science
Year:
1994
Type:
Article
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