Detection of immunoglobulins on bacterial surface by laser flow cytometry: analysis between Haemoplhilus influenzae type b and Vibrio cholerae 01 of healthy mother-full term newborn sera
Arch. med. res
;
27(1): 67-70, 1996. tab, ilus
Article
in English
| LILACS
| ID: lil-200293
ABSTRACT
The identification of human IgG immunoglobulins on the surface of Vibrio cholerae O1, and Haemophilus influenzae type b microorganisms was assessed via a flow cytometric technique. A group of 31 healthy mother-full term nerwborn duo sera from a non-endemic cholera area was assayed. The sera of mothers and full-term newborns against both microorganisms were compared. The mean fluorescent intensity of the samples was not different at the 0.05 significance level by paired t-test. On the other hand, the immunoglobulins of newborn and mothers for V. cholerae O1 was notably lower when compared with H. influenzae type b microorganisms (p<0.05 by paried t-test, t=-5.570 for mother' sera, and t=-7.496 for the sera of the newborns). These data provide circumstantial evidence that LFC techique would be useful on bacteria-related serology
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Index:
LILACS (Americas)
Main subject:
Bacterial Outer Membrane Proteins
/
Vibrio cholerae
/
Immunoglobulin G
/
Immunoglobulins
/
Serologic Tests
/
Haemophilus influenzae
/
Maternal and Child Health
/
Brucella melitensis
/
Flow Cytometry
Type of study:
Diagnostic study
Limits:
Female
/
Humans
Language:
English
Journal:
Arch. med. res
Journal subject:
Medicine
Year:
1996
Type:
Article
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