Different arrangement of human papillomavirus E2 binding sites distinguishes cutaneous types from those associated with mucosal lesions
Arch. med. res
;
27(3): 389-94, 1996. ilus
Article
in English
| LILACS
| ID: lil-200340
ABSTRACT
High-risk-type human papillomavirus DNA sequences are found in a high percentage of carcinomas from the uterine-cervix, with the viral E1-E2 gene region usually disrupted and the E6 and E7 oncoproteins consistently expressed. The E2 protein is known to repress early transcription from genital HPV promoters having a proximal E2 binding site (2BS) close to the TATA box. On the contrary, the E2 protein activates cutaneous early promotes, the E2 protein activates cutaneous early promoters having a longer distance between these sites. Using an in vivo approach we analyzed the regulation, by the BPV-1 E2 protein, of a natural HPV-18 promoter where proximal E2BS were placed at variable positions relative to the TATA box, and of heterologous promoters where E2BS was placed upstream of any other known DNA-binding elements. Our results confirm that the E2 protein represses or activates HPV early gene transcription depending on the distance between the TATA box and the proximal E2BS
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Index:
LILACS (Americas)
Main subject:
Papillomaviridae
/
Binding Sites
/
Uterine Cervical Dysplasia
/
Cervix Mucus
/
Gene Expression Regulation
Type of study:
Risk factors
Language:
English
Journal:
Arch. med. res
Journal subject:
Medicine
Year:
1996
Type:
Article
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