Alanine aminotransferase activity in mammary tissue, muscle and liver of dam rat during lactation and weaning

ABSTRACT

Transamination reaction is the first step in the catabolism of most of the L-amino acids. Alanine is an important molecule in the inter-organ nitrogen transport, conveying them from muscle to the liver Amino groups from this amino acid are generally first transferred to alfa-ketoglutarate in the cytosol of liver cells to form glutamate and leaving behing the corresponding alfa-keto acid analog. Measurements of the alanine aminotransferase (EC2.6.1.2.) activity were compared in liver, mammary gland an skeletal muscle in virgin, lactating and weaning dam rats. In this study liver was the principal tissue involved in alanine transamination, while muscle showed a reduction in the enzyme activity during lactation. Results indicate an increase in alanine aminotransferase activity in the mammary gland during lactation and weaning when compared with virgin rats. This suggest that mammary gland during lactation is an importan extra-hepatic tissue involved in the metabolism of alanine and probably shunted into hte pathways for amino group metabolism in terms of nitrogen economy