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Recombination of epithelial root sheath and dental papilla cells in vitro
Arch. med. res ; 27(4): 573-7, 1996. ilus
Article in English | LILACS | ID: lil-200365
Responsible library: MX1.1
RESUMO
Hertwing's epithelial root sheath (HERS) cells were isolated and recombined with ectomesenchymal cells in vitro utilizing extracellular matrix components as substrate. After 14 days in culture, HERS cells were differnetiated and exhibited a stratified organization. These features resembled those observed in vivo as epithelial rests of Malassez. A mineralization process was also present in HERS cells, in which calcium salts were deposited. This mineralization was correlated with the strong immunoexpression of osteopontin by HERS. The results obtained add support to the possible role of HERS in the secretion of Hypocalcified material on the root during early cementogenesis
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Index: LILACS (Americas) Main subject: Tooth Calcification / Calcification, Physiologic / Cell Differentiation / Dental Cementum / Dental Papilla / Epithelium / Mice, Inbred BALB C Limits: Animals Language: English Journal: Arch. med. res Journal subject: Medicine Year: 1996 Type: Article

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Index: LILACS (Americas) Main subject: Tooth Calcification / Calcification, Physiologic / Cell Differentiation / Dental Cementum / Dental Papilla / Epithelium / Mice, Inbred BALB C Limits: Animals Language: English Journal: Arch. med. res Journal subject: Medicine Year: 1996 Type: Article