Isoenzyme analysis of Arthrobotrys, a nematode-trapping fungus
Braz. j. med. biol. res
;
30(10): 1149-52, Oct. 1997. ilus
Article
in English
| LILACS
| ID: lil-201529
RESUMO
Extraction and isoenzyme analysis of four isolates of Arthrobotrys including A. musiformis, A. robusta and A. conoides were conducted. Among the 14 enzymes studied by starch gel electrophoresis, using morpholine-citrate as gel/electrode buffer, the following nine enzymes showed interpretable banding patterns alpha-esterase, fumarase, hexokinase, isocitrate dehydrogenase, leucine aminopeptidase, malate dehydrogenase, 6-phosphogluconate dehydrogenase, phosphoglucomutase and phosphoglucoisomerase. All isolates studied displayed typical isoenzyme phenotypes for each species. Two isolates of A. conoides differed in their alpha-isoesterase banding patterns, but no differences were observed for the other enzymes. The assay was satisfactory for enzyme extraction and resolution of Arthrobotrys and could be used in future taxonomic and genetic studies of this organism.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
In Vitro Techniques
/
Fungi
/
Isoenzymes
/
Nematoda
Limits:
Animals
Language:
English
Journal:
Braz. j. med. biol. res
Journal subject:
Biology
/
Medicine
Year:
1997
Type:
Article
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