Production of an effective anti-Bothrops-tetanus mixed hyperimmune serum of equine origin
J. venom. anim. toxins
;
4(1): 70-8, 1998. tab
Article
in English
| LILACS
| ID: lil-201616
RESUMO
The present investigation reveals the possibility of simultaneous immunization of horses with Bothrops or Crotalus snake venoms and Tetanus antigens for the production of anti-Bothrops-Tetanus or anti-Crotalus-Tetanus mixed serum, with high titers of the respective specific antibodies. Bothrops antivenoms with an average neutralizing titer of 4.16 mg venom/ml were obtained from plasma of horses with titers lower than 0.5 mg venom/ml when Tetanus antigens were not used. This suggests the existence of a synergism between Bothrops venoms and Tetanus antigens in the stimulation of the antibody response. The pooled plasma of the animal had a neutralizing titer of 21.0 mg/ml reference Bothrops venoms and 3,300 IU/ml to Tetanus antigens after purification by enzymatic digestion and ammonium sulphate precipitation. These experiments lead us to conclude that Bothrops envenomation therapy can be successfully performed using Anti-Bothrops-Tetanus serum also serving as Tetanus prophylaxis. anti-Crotalus-Tetanus serum can also be produced, although it is not of medical interest as Crotalus envenomation rarely results in local necrotizing lesions.
Full text:
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Index:
LILACS (Americas)
Main subject:
Snake Venoms
/
Tetanus
/
Antivenins
/
Immunization
/
Clostridium tetani
/
Horses
Limits:
Animals
Language:
English
Journal:
J. venom. anim. toxins
Journal subject:
Toxicology
Year:
1998
Type:
Article
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