Systemic modulation of peripheral eosinophilia (air pouch model) in Schistosoma mansoni infection
Mem. Inst. Oswaldo Cruz
;
92(supl.2): 165-72, Dec. 1997. ilus, tab, graf
Article
in English
| LILACS
| ID: lil-202028
RESUMO
Schistosoma mansoni infection induces in their hosts a marked and sustained eosinophilia, which is influenced or modulated by complex mechanisms, that vary according to the phase of infection. To address this phenomenon, we used the air pouch (AP) model in control and infected Swiss webster mice, analyzing the cellular, tissue response and local expression of adhesion molecules [CD18 (ß2-chain), CD44, ICAM-1 (CD54), L-selectin (CD62L), CD49d (alpha4-chain), LFA1 (CD11a)]. Infected animals were studied at 3 (pre-oviposition phase), 7 (acute phase), and 14 (chronic phase) weeks after infection (5-6 mice/period of infection). Normal mice were age-matched. Results showed that after egg stimulation, compared with matched controls, the infected mice, at each point of infection, showed a lower eosinophil response in the acute (7 weeks) and chronic phase (14 weeks) of infection. However, when the infected mice were in pre-oviposition phase (3 weeks) their eosinophil response surpassed the control ones. In the AP wall of infected mice, a significant decrease in the expression of ICAM-1 and CD44 in fibroblastic-like cells and a reduction in the number of CD18 and CD11 a in migratory cells were observed. The other adhesion molecules were negative or weakly expressed. The results indicated that in the air pouch model, in S. mansoni-infected mice (1) eosinophil response is strikingly down-regulated, during the acute ovular phase; (2) in the pre-oviposition phase, in contrast, it occurs as up-regulatory modulation of eosinophil responses, in which the mechanisms are completely unknown; (3) in the chronic phase of the infection, the down modulation of eosinophil response is less pronounced; (4) Down-regulation of adhesion molecules, specially of ICAM-1 appear to be associated with the lower eosinophil response.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Schistosoma mansoni
/
Eosinophilia
Limits:
Animals
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
1997
Type:
Article
/
Congress and conference
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