In situ hybridization and immunoperoxidase for quick detection and identification of leishmania braziliensis in tissue
Parasitol. día
;
20(3/4): 86-90, jul.-dic. 1996. ilus, tab
Article
in English
| LILACS
| ID: lil-202465
ABSTRACT
The combined used of in situ hybridization and immunoperoxidase techniques applied to histological sections of the biopsy allowed an increment of the efficiency in the detection of Leishmania diagnosis.The molecular basis for DNA diagnosis is to allow labelled single-stranded species or strain-specific DNA sequences, selected from well characterised reference species, to find and hybridize with homologous DNA from or in, the unknown isolates of parasites. The use of immunoperoxidase assay was useful in disclosing the antigen pathways and revealed amastigotes and antigenic material in various morphological patterns
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Index:
LILACS (Americas)
Main subject:
Leishmania braziliensis
/
Immunoenzyme Techniques
/
In Situ Hybridization
Type of study:
Diagnostic study
Limits:
Animals
Country/Region as subject:
South America
/
Brazil
Language:
English
Journal:
Parasitol. día
Journal subject:
Parasitology
Year:
1996
Type:
Article
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