Determinación del contenido de ADN en tumores de glándulas salivales / Determination of DNA content of salivary gland tumors

RESUMO

Background: DNA content determination is a useful tool in the characterization of different malignant tumors. Aim: to measure DNA content in cells of salivary gland tumors as adjunct to histological diagnosis, correlatting morphologic and biological features of these tumors. Material and methods: from the archives of the pathology service of a general hospital, 21 salivary gland tumors, 15 pleomorphic adenomas, 3 mucoepidermoid carcinomas and 3 cystic adenoid carcinomas were seleced. DNA content was determined in the histological samples using a flow cytometric DNA analysis. Results: all pleomorphic adenomas had a normal or diploid DNA content. Fifty percent of malignant tumors had an aneuploid DNA content (1 mucoepidermoid carcinoma and 2 cystic adenoid carcinomas). Conclusions: DNA determination may help in the histological diagnosis of salivary gland tumors. The presence of aneuploidy suggests malignity