Purification of bovine pancreatic glucagon as a by-product of insulin production
Braz. j. med. biol. res
;
30(12): 1421-6, Dec. 1997. tab, graf
Article
in English
| LILACS
| ID: lil-212585
RESUMO
A process for purifying bovine pancreatic glucagon as a by-product of insulin production is described. The glucagon-containing supernatant from the alkaline crystallization of insulin was precipitated using ammonium sulfate and isoelectric precipitation. The isoelectric precipitate containing glucagon was then purified by ion-exchange chromatography on Q-Sepharose FF, gel filtration on Sephadex G-25 and ion-exchange chromatography on S-Sepharose FF. A pilot scale test was performed with a recovery of 87.6 percent and a purification factor of 8.78 for the first chromatographic step, a recovery of 75.1 percent and a purification factor of 3.90 for the second, and a recovery of 76.2 percent and a purification factor of 2.36 for the last one. The overall yield was 50 percent, a purification factor of 80.8 was obtained and the fraction containing active glucagon (suitable for pharmaceutical preparations) was 84 percent pure as analyzed by HPLC.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Pancreas
/
Glucagon
/
Insulin
Limits:
Animals
Language:
English
Journal:
Braz. j. med. biol. res
Journal subject:
Biology
/
Medicine
Year:
1997
Type:
Article
/
Congress and conference
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