High-performance liquid chromatographic determination of norepinephrine, epinephrine and dopamine in human foetal adrenal gland
La Habana; s.n; 1994. 4 p. tab, graf.
Non-conventional
in English
| LILACS
| ID: lil-223633
RESUMO
The purpose of this work to characterize the human foetal adrenal gland (HFAG) by studying norepinephrine (NE), epinephrine (E), and dopamine (DA) levels in foetuses with ages ranging from 10 to 18 weeks, as a source of chromaffin cells for basic and clinical research, in the treatment of Parkinson's disease. The HFAGs were obtained from ten abortions with foetal ages ranging from 10-12 (n=7) to 13-18 (n=3) weeks. For the simultaneous detection of NE, E, and DA a high-performance liquid chromatographic (HPLC) procedure with electrochemical detection was employed. The concentration found (ng/mg wet weight) were 24.24ñ7.06 for NE, 3.56ñ2.97 for E and 0.45ñ0.27 for DA at 10-12 weeks and 14.91ñ9.94 for NE, 8.18ñ8.79 for E and 0.16ñ0.05 for DA at 13-18 weeks. The DAE ratio present in HFAG between 10 and 12 weeks was 100 times higher than that reported by other authors in adult adrenal medulla
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Index:
LILACS (Americas)
Main subject:
Parkinson Disease
/
Dopamine
/
Epinephrine
/
Norepinephrine
/
Chromatography, Liquid
Limits:
Humans
Language:
English
Year:
1994
Type:
Non-conventional
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