Basic surface properties of mononuclear cells from Didelphis marsupialis
Mem. Inst. Oswaldo Cruz
;
93(6): 807-14, Nov.-Dec. 1998. ilus, tab
Article
in English
| LILACS
| ID: lil-223886
ABSTRACT
The electrostatic surface charge and surface tension of mononuclear cells/monocytes obtained from young and adult marsupials (Didelphis marsupialis) were investigated by using cationized ferritin and colloidal iron hydroxyde, whole cell electrophoresis, and measuments of contact angles. Anionic sites were found distributed throughout the entire investigated cell surfaces. The results revealed that the anionic character of the cells is given by electrostatic charges corresponding to -18.8 mV (cells from young animals) and -29.3 mV (cells from adult animals). The surface electrostatic charge decreased from 10 to 65.2 per cent after treatment of the cells with each one of trypsin, neuraminidase and phospholipase C. The hydrophobic nature of the mononuclear cell surfaces studied by using the contact angle method revealed that both young and adult cells posses cell surfaces of high hidrofilicity since the angles formed with drops of saline water were 42.5º and 40.8º, respectively. Treatment of the cells, with trypsin or neuraminidase rendered their surfaces more hydrophobic, suggesting that sialic acid-containing glycoproteins are responsable for most of the hydrophilicity observed in the mononuclear cell surfaces from D. marsupialis.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Opossums
/
Monocytes
/
Cells
Limits:
Animals
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
1998
Type:
Article
Similar
MEDLINE
...
LILACS
LIS