Detection of point mutations by non-isotopic single strand conformation polymorphism
Braz. j. med. biol. res
;
32(1): 55-8, Jan. 1999. graf, tab
Article
in English
| LILACS
| ID: lil-226213
RESUMO
We have developed a procedure for nonradioactive single strand conformation polymorphism analysis and applied it to the detection of point mutations in the human tumor suppressor gene p53. The protocol does not require any particular facilities or equipment, such as radioactive handling, large gel units for sequencing, or a semiautomated electrophoresis system. This technique consists of amplification of DNA fragments by PCR with specific oligonucleotide primers, denaturation, and electrophoresis on small neutral polyacrylamide gels, followed by silver staining. The sensitivity of this procedure is comparable to other described techniques and the method is easy to perform and applicable to a variety of tissue specimens
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Stomach Neoplasms
/
Uterine Cervical Neoplasms
/
Genes, p53
/
Point Mutation
/
Polymorphism, Single-Stranded Conformational
/
Head and Neck Neoplasms
Type of study:
Diagnostic study
Limits:
Humans
Language:
English
Journal:
Braz. j. med. biol. res
Journal subject:
Biology
/
Medicine
Year:
1999
Type:
Article
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