Molecular mechanisms of angiogenesis: experimental models define cellular trafficking of FGF-1
P. R. health sci. j
;
15(3): 179-86, Sept. 1996.
Article
in English
| LILACS
| ID: lil-228509
ABSTRACT
Numerous studies have established that stimulation of cell growth by members of the fibroblast growth factor (FGF) family of polypeptides is dependent upon an extracellular pathway. Acidic FGF (FGF-1), however, lacks a classical signal sequence for secretion, thereby making it difficult to evaluate regulation of biological activity by this growth factor. Efforts in this laboratory have utilized molecular techniques of retrovirology and transgenic modeling to introduce cDNA sequences encoding either an intracellular or extracellular form of FGF-1 into primary diploid cells to examine trafficking and compartmentalization of FGF-1. Several lines of evidence obtained from these models provide a compelling argument that the stimulation of FGF-1-associated cellular transformation is restricted to an extracellular, receptor-mediated pathway, involving protein tyrosine phosphorylation and nuclear localization. In addition, an unconventional secretion pathway for intracellular FGF-1 has been identified that involves mechanisms associated with oxidative stress
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Index:
LILACS (Americas)
Main subject:
Signal Transduction
/
Fibroblast Growth Factor 1
/
Neovascularization, Physiologic
Type of study:
Prognostic study
Limits:
Animals
Language:
English
Journal:
P. R. health sci. j
Journal subject:
Medicine
Year:
1996
Type:
Article
Affiliation country:
Brazil
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