Identification of GTPase genes in the protozoa parasites Trypanosoma cruzi and Leishmania amazonensis
Biol. Res
; 26(1/2): 3-9, 1993. ilus
Article
in En
| LILACS
| ID: lil-228613
Responsible library:
BR1.1
ABSTRACT
A PCR based assay was designed in order to amplify putative ras gene sequences of the GTPase superfamily eventually present in Leishmania amazonensis and Trypanosoma cruzi. A set of primers corresponding to the conserved motifs G1 and G3 of the GTP binding proteins was synthesized. Sequencing of six PCR products (three from Leishmania and three from Trypanosoma) identified, however, two other different GTPases. The 270 bp L. amazonensis clone, pLef-11, shared an amino acid identity of around 80 percent with an eukaryotic elongation factor 1a of protein synthesis. On the other hand, the 168 bp T. cruzi clone, pTCr1, demonstrated over 60 percent amino acid identity to ras-related proteins of the rab-YPT-SEC4 family involved in control of vesicular traffic. To our knowledge, this is the first report of GTP binding protein genes in trypanosomatids
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Index:
LILACS
Main subject:
Trypanosoma cruzi
/
Leishmania mexicana
/
Genes, Protozoan
/
GTP Phosphohydrolases
Type of study:
Diagnostic_studies
/
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
Biol. Res
Journal subject:
BIOLOGIA
Year:
1993
Type:
Article