The separation and identification of picomole amounts of intermediates of glucose metabolism by high performance liquid chromatography on pellicular resins
Biol. Res
;
25(2): 73-8, 1992. graf
Article
in English
| LILACS
| ID: lil-228636
ABSTRACT
A column (CarboPac PA1, Dionex) containing an anion-exchange pellicular resin was used for the separation of phosphoryl-hexoses derived from labeled glucose microinjected into individual frog oocytes or from cultures of Escherichia coli. Intermediates were identified by a) comparison of retention times with those of authentic commercial compounds; b) the use of internal labeled standards; c) incubation of samples with specific enzymes and noting the disappearance of one radioactive peak and appearance of another at a new retention time
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Index:
LILACS (Americas)
Main subject:
Chromatography, High Pressure Liquid
/
Glucose
Type of study:
Diagnostic study
/
Prognostic study
Limits:
Animals
Language:
English
Journal:
Biol. Res
Journal subject:
Biology
Year:
1992
Type:
Article
Affiliation country:
Brazil
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