Dot enzyme-linked immunosorbent assay (dot-ELISA) for schistosomiasis diagnosis using dacron as solid-phase / Dot-ELISA (dot enzyme-linked immunosorbent assay), utilizando o dacron como suporte sólido para o diagnóstico da esquistossomose
Rev. Soc. Bras. Med. Trop
;
32(2): 139-43, mar.-abr. 1999. tab
Article
in English, Portuguese
| LILACS
| ID: lil-235183
RESUMO
Dacron and nitrocellulose were evaluated as matrices for the dot enzyme linked immunosorbent assay (dot-ELISA) for schistosomiasis and compared to indirect immunofluorescence (IMF). Titration of sera from 18 schistosomiasis patients against soluble worm antigen preparation (SWAP) was carried out and sera from healthy individuals from non-endemic areas were used as controls. The IMF was less sensitive than the dot-ELISAs, although the difference was not statistically significant (p > 0.05). The dot-ELISA based on nitrocellulose was as sensitive as that using dacron. Stability did not differ between nitrocellulose and dacron. Specificity was lower when dacron was used than when nitrocellulose was used, although the difference was not statistically significant (p > 0.05). In conclusion, this work showed that nitrocellulose and dacron performed similarly in dot-ELISA, suggesting that they may be used altenatively in population surveillance in endemic areas
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Schistosoma mansoni
/
Schistosomiasis mansoni
/
Antibodies, Helminth
/
Collodion
/
Micropore Filters
/
Antigens, Helminth
Type of study:
Diagnostic study
Limits:
Humans
Language:
English
/
Portuguese
Journal:
Rev. Soc. Bras. Med. Trop
Journal subject:
Tropical Medicine
Year:
1999
Type:
Article
Affiliation country:
Brazil
Similar
MEDLINE
...
LILACS
LIS