Partial protection of mice against Trypanosoma cruzi after immunizing with the TcY 72 antigenic preparation
Mem. Inst. Oswaldo Cruz
;
94(2): 167-72, Mar.-Apr. 1999. ilus, tab, graf
Article
in English
| LILACS
| ID: lil-239012
RESUMO
A 72 kDa Trypanosoma cruzi glycoprotein recognized by the 164C11 monoclonal antibody (IgM isotype) was purified by preparative electrophoresis. The antigenic preparation obtained, named TcY 72, was used to immunize C57Bl/10 mice. The following results were observed after immunization (1) induction of higher titres of IgG than IgM antibodies, as evaluated by indirect immunofluorescence; (2) significant DTH after infection of epimastigotes in mice footpads (3) peak parasitemia in immunized mice was significantly reduced and animals were negative by 13 days post-infection, although the mice still succumb to infection (4) the phenotypic analysis of spleen cell populations showed a decrease in the CD4/CD8 ratio in immunized mice. Taken as a whole, these findings indicate that TcY 72 is immunogenic and potentially important for protective immunity.
Full text:
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Index:
LILACS (Americas)
Main subject:
Trypanosoma cruzi
/
Antibodies, Protozoan
/
Immunization, Passive
/
Chagas Disease
/
Mice
/
Antigens, Protozoan
Limits:
Animals
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
1999
Type:
Article
Affiliation country:
Brazil
/
France
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