A dhfr-ts- Leishmania major Knockout Mutant Cross-protects against Leishmania amazonensis
Mem. Inst. Oswaldo Cruz
;
94(4): 491-6, July-Aug. 1999.
Article
in English
| LILACS
| ID: lil-241561
RESUMO
E10-5A3 is a dhfr-ts- Leishmania major double knockout auxotrophic shown previously to induce substantial protection against virulent L. major infection in both genetically susceptible and resistant mice. We investigated the capacity of dhfr-ts- to protect against heterologous infection by L. amazonensis. The degree of protection was evaluated by immunization of BALB/c or C57BL/6 mice with E10-5A3, followed by L. amazonensis challenge. Whether immunized by subcutaneous (SC) or intravenous (IV) inoculation, susceptible and resistant mice displayed a partial degree of protection against challenge with virulent L. amazonensis. SC-immunized BALB/c mice developed lesions 40 to 65 percent smaller than non immunized mice, while IV immunization led to protection ranging from 40 to 75 percent in four out of six experiments compared to non immunized animals. The resistant C57BL/6 mice displayed comparable degrees of protection, 57 percent by SC and 49 percent by IV immunization. Results are encouraging as it has been previously difficult to obtain protection by SC vaccination against Leishmania, the preferred route for human immunization
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Thymidylate Synthase
/
Protozoan Vaccines
/
Leishmaniasis, Cutaneous
/
Leishmania major
/
Antigens, Protozoan
Limits:
Animals
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
1999
Type:
Article
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