A simple procedure for rehybridization of nuclei analyzed previously by fish
Genet. mol. biol
;
22(2): 173-5, jun. 1999. ilus
Article
in English
| LILACS
| ID: lil-242196
ABSTRACT
We present a simple procedure for interphase fluorescence in situ rehybridization (FISH). This procedure was used to evaluate fresh prostate tumor from needle biopsy specimens. Digoxigenin-labeled DNA probes were hybridized onto nuclei which had been previously investigated by FISH using biotin-labeled DNA probes. This method makes it possible to reanalyze the same nuclei with different centromeric DNA probes and is useful in cases where a limited number of slides are available.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Prostatic Neoplasms
/
Sequence Analysis, DNA
/
Nucleic Acid Hybridization
Limits:
Humans
/
Male
Language:
English
Journal:
Genet. mol. biol
Journal subject:
Genetics
Year:
1999
Type:
Article
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