Critical electrolyte concentration of spermatozoal chromatin containing histone H1 variants
Genet. mol. biol
;
22(2): 197-200, jun. 1999. tab
Article
in English
| LILACS
| ID: lil-242200
ABSTRACT
The critical electrolyte concentrations (CEC) of sperm chromatin from animal species known or suspected to contain histone H1 variants were compared by examining the affinity of their DNA-protein complexes for toluidine blue in the presence of Mg2+. Bullfrog, sea urchin, bee and bumblebee spermatozoa were studied. The CEC for Rana catesbeiana and two sea urchin species were similar to that of histone H5-containing chromatin from chicken erythrocytes, thus confirming the biochemical and structural similarities of these DNA-protein complexes. The CEC for bees and the bumblebee, Bombus atratus, showed no particular phylogenetic relationship. We concluded that the CEC of histone H1-containing sperm cell chromatin is a useful indicator of variability in DNA-protein complexes but is of little phylogenetic value.
Full text:
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Index:
LILACS (Americas)
Main subject:
Spermatozoa
/
Chromatin
/
Histones
/
Electrolytes
Limits:
Animals
Language:
English
Journal:
Genet. mol. biol
Journal subject:
Genetics
Year:
1999
Type:
Article
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